DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Product Name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- CAS No.
- 199593-09-4
- Chemical Name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Synonyms
- (2R,3R,4R,5R)-5-(4-Acetamido-2-oxopyrimidin-1(2H)-yl)-2-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-methoxytetrahydrofuran-3-yl (2-cyanoethyl) diisopropylphosphoramidite;Cytidine Impurity 14;2’-OMe -dC(Ac) Amidite;N4-Ac-5'-O-DMT-2'-OMe-C-CE;N4-Ac-5'-O-DMT-2'-OMe-C-CE;2'-OMe-Ac-C Phosphoramidite;Ac-2'-Ome-rC Phosphoramidite;2'-OMe-Ac-C Phosphoramidite;2'-OMe-C(Ac) Phosphoramidite;2'-OMe-N-Ac Cytidine?Amidite
- CBNumber
- CB21871625
- Molecular Formula
- C42H52N5O9P
- Formula Weight
- 801.86
- MOL File
- 199593-09-4.mol
DMT-2′O-Methyl-rC(ac) Phosphoramidite Property
- Melting point:
- 102 - 104°C
- storage temp.
- Sealed in dry,2-8°C
- solubility
- DMSO (Slightly), Methanol (Slightly)
- form
- powder
- pka
- 10.18±0.20(Predicted)
- color
- white to off-white
- InChI
- InChI=1/C42H52N5O9P/c1-28(2)47(29(3)4)57(54-26-12-24-43)56-38-36(55-40(39(38)52-8)46-25-23-37(44-30(5)48)45-41(46)49)27-53-42(31-13-10-9-11-14-31,32-15-19-34(50-6)20-16-32)33-17-21-35(51-7)22-18-33/h9-11,13-23,25,28-29,36,38-40H,12,26-27H2,1-8H3,(H,44,45,48,49)/t36-,38-,39-,40-,57?/s3
- InChIKey
- WNWUMIPFLOKTEZ-UAQIPLLRSA-N
- SMILES
- O(C(C1=CC=C(OC)C=C1)(C1=CC=C(OC)C=C1)C1=CC=CC=C1)C[C@H]1O[C@@H](N2C=CC(NC(C)=O)=NC2=O)[C@H](OC)[C@@H]1OP(N(C(C)C)C(C)C)OCCC#N |&1:25,27,39,42,r|
Safety
- Safety Statements
- 24/25
- WGK Germany
- 3
- HS Code
- 29349990
Hazard and Precautionary Statements (GHS)
- Symbol(GHS)
-
- Signal word
- Warning
- Hazard statements
-
H315Causes skin irritation
H319Causes serious eye irritation
H335May cause respiratory irritation
- Precautionary statements
-
P261Avoid breathing dust/fume/gas/mist/vapours/spray.
P305+P351+P338IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.
N-Bromosuccinimide Price
- Product number
- C213180
- Product name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Purity
- configured for PerkinElmer, configured for Polygen
- Packaging
- 1G
- Price
- $125
- Updated
- 2024/03/01
- Product number
- C213100
- Product name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Purity
- ≥99% (31P-NMR)
- Packaging
- 100g
- Price
- $6540
- Updated
- 2024/03/01
- Product number
- C213100
- Product name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Purity
- ≥99% (31P-NMR)
- Packaging
- 500g
- Price
- $32550
- Updated
- 2024/03/01
- Product number
- C213180
- Product name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Purity
- configured for PerkinElmer, configured for Polygen
- Packaging
- 0.5g
- Price
- $92.7
- Updated
- 2024/03/01
- Product number
- C213130
- Product name
- DMT-2′O-Methyl-rC(ac) Phosphoramidite
- Purity
- configured for ABI
- Packaging
- 1g
- Price
- $84.7
- Updated
- 2024/03/01
DMT-2′O-Methyl-rC(ac) Phosphoramidite Chemical Properties,Usage,Production
Uses
2''-OMe-Ac-C Phosphoramidite (CAS# 199593-09-4) is a nucleotide used in the solid-phase synthesis of phosphorothioate oligonucleotides using sulfurization byproducts for in situ capping.
Application
2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rC(ac) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:
Diagnostic probes
Aptamer and ribozyme development
Mixed 2′O-Methyl-RNA/DNA antisense molecules
General Description
DMT-2′O-Methyl-rC(ac) Phosphoramidite is one of the 2′O-Methyl RNA monomers that feature methoxy groups at the 2′-position. Themethoxy groups are perfectly stable in all conditions employed in theassembly of oligonucleotides by automated phosphoramidite synthesis, andin all standard alkaline deprotection conditions.Its key features include:
High yield of crude oligonucleotides
Compatible with DNA synthesis
Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides
Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradation
Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers)
2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry
DMT-2′O-Methyl-rC(ac) Phosphoramidite Preparation Products And Raw materials
Raw materials
Preparation Products
DMT-2′O-Methyl-rC(ac) Phosphoramidite Suppliers
View Lastest Price from DMT-2′O-Methyl-rC(ac) Phosphoramidite manufacturers
- Product
- 2'-OMe-Ac-C CE Phosphoramidite 199593-09-4
- Price
- US $100.00/kg
- Min. Order
- 0.009999999776482582kg
- Purity
- 99%
- Supply Ability
- 5tons
- Release date
- 2023-03-21
- Product
- N4-Ac-5'-O-DMT-2'-OMe-C-CE 199593-09-4
- Price
- US $0.00/mg
- Min. Order
- 100mg
- Purity
- ≥98%
- Supply Ability
- 20tons
- Release date
- 2023-03-09
- Product
- N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-methylcytidine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite 199593-09-4
- Price
- US $0.00-0.00/kg
- Min. Order
- 1kg
- Purity
- 98%
- Supply Ability
- 1Ton
- Release date
- 2022-09-23